Chromatography continues to be an essential technology for the purification of biomolecules. Pall’s recent acquisition of BioSepra products complements our current technology portfolio and expands our offering to include resins. The BioSepra line of chromatography resins greatly simplifies protein purification and fractionation. These broad lines of chromatography products exhibit superior performance and are useful for affinity, ion exchange, size exclusion and hydrophobic interaction (HCIC) chromatography. Unique mixed-mode BioSepra products also provide solutions to current sample preparation challenges such as detergent removal and antibody purification.

• Versatile product line – Pall bottled resins can be used for small and large sample sizes involving single use or high throughput methods of purification.
• High binding capacities and fast flow rates – By tailoring attributes to specific applications, Pall chromatography resins exhibit the highest performance characteristics possible while ensuring reliable, reproducible protein isolation.

The HyperD® line of ion exchange resins shows high dynamic binding capacity (50-110 mg BSA/mL of resin for resins tested here). Dynamic binding capacity is measured in a 1 mL packed column by pumping BSA (anion) or lysozyme (cation) at 5 mg/mL in a suitable binding buffer until the column capacity is exceeded. The capacity is then calculated by estimating the volume of protein required to achieve this “breakthrough” and expressed as mg/mL media volume. Anion and cation chemistries are available in both 80-90 µm (HyperD F resins) and 20 µm (HyperD 20 resins) particle sizes for improved resolution.

Flow Rate (mL/min.)

Media	1	5	10
HyperD Q-20	106.0 mg/mL	91.5 mg/mL	82.5 mg/mL
HyperD F DEAE	101.5 mg/mL	87.5 mg/mL	77.5 mg/mL
HyperD F S	80.5 mg/mL	61.5 mg/mL	53.5 mg/mL
HyperD S-20	98.0 mg/mL	89.5 mg/mL	83.5 mg/mL
HyperD F CM	108.0 mg/mL	87.5 mg/mL	73.5 mg/mL

SDR HyperD resin binds detergents used in viral inactivation processes (e.g., TnBP and Triton™ X-100), as well as other common detergents used in protein procedures (e.g., CHAPS, SDS, ASB14). High recovery of proteins (exclusion limit 10 kDa) is obtained. This product exhibits high adsorption capacity for small hydrophobic molecules and is stable in acidic, polar organic and oxidizing solutions.

Detergent Triton (DBC = 60-80 mg/mL)	Protein Solution IgG	Protein Solution AT-III	Protein Solution Bovine Serum
Initial Conc. (ppm)	10,000	10,000	10,000
Final Conc. (ppm)	< 10	< 10	340
Removal Efficiency	> 99.9%	> 99.9%	> 95.2%

For specifications and ordering information on the products presented above, see:

• Q, S, DEAE, CM Ceramic HyperD® Ion Exchange Sorbents, Laboratory Scale Volumes
• SDR HyperD® Solvent-Detergent Removal Chromatography Resin, Laboratory Scale Volumes

Or, get information on Pall's entire line of Chromatography Products.